Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.

TitleDiet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.
Publication TypeJournal Article
Year of Publication2008
AuthorsTurnbaugh, PJ, Bäckhed, F, Fulton, L, Gordon, JI
JournalCell Host Microbe
Volume3
Issue4
Pagination213-23
Date Published2008 Apr 17
ISSN1934-6069
KeywordsAnimals, Bacteria, Carbohydrate Metabolism, Carbohydrates, Cecum, Diet, Disease Models, Animal, Energy Metabolism, Eubacterium, Fats, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Molecular Sequence Data, Obesity, RNA, Bacterial, RNA, Ribosomal, 16S, Specific Pathogen-Free Organisms, Tenericutes
Abstract

We have investigated the interrelationship between diet, gut microbial ecology, and energy balance using a mouse model of obesity produced by consumption of a prototypic Western diet. Diet-induced obesity (DIO) produced a bloom in a single uncultured clade within the Mollicutes class of the Firmicutes, which was diminished by subsequent dietary manipulations that limit weight gain. Microbiota transplantation from mice with DIO to lean germ-free recipients promoted greater fat deposition than transplants from lean donors. Metagenomic and biochemical analysis of the gut microbiome together with sequencing and metabolic reconstructions of a related human gut-associated Mollicute (Eubacterium dolichum) revealed features that may provide a competitive advantage to members of the bloom in the Western diet nutrient milieu, including import and processing of simple sugars. Our study illustrates how combining comparative metagenomics with gnotobiotic mouse models and specific dietary manipulations can disclose the niches of previously uncharacterized members of the gut microbiota.

DOI10.1016/j.chom.2008.02.015
Alternate JournalCell Host Microbe
PubMed ID18407065
PubMed Central IDPMC3687783
Grant ListDK30292 / DK / NIDDK NIH HHS / United States
DK70977 / DK / NIDDK NIH HHS / United States
P01 DK078669 / DK / NIDDK NIH HHS / United States
P30 DK056341 / DK / NIDDK NIH HHS / United States
P30 DK056341-07 / DK / NIDDK NIH HHS / United States
P30 DK056341-08 / DK / NIDDK NIH HHS / United States
R37 DK030292 / DK / NIDDK NIH HHS / United States