Gut microbial succession follows acute secretory diarrhea in humans.

TitleGut microbial succession follows acute secretory diarrhea in humans.
Publication TypeJournal Article
Year of Publication2015
AuthorsDavid, LA, Weil, A, Ryan, ET, Calderwood, SB, Harris, JB, Chowdhury, F, Begum, Y, Qadri, F, LaRocque, RC, Turnbaugh, PJ
JournalMBio
Volume6
Issue3
Paginatione00381-15
Date Published2015
ISSN2150-7511
Abstract

UNLABELLED: Disability after childhood diarrhea is an important burden on global productivity. Recent studies suggest that gut bacterial communities influence how humans recover from infectious diarrhea, but we still lack extensive data and mechanistic hypotheses for how these bacterial communities respond to diarrheal disease and its treatment. Here, we report that after Vibrio cholerae infection, the human gut microbiota undergoes an orderly and reproducible succession that features transient reversals in relative levels of enteric Bacteroides and Prevotella. Elements of this succession may be a common feature in microbiota recovery from acute secretory diarrhea, as we observed similar successional dynamics after enterotoxigenic Escherichia coli (ETEC) infection. Our metagenomic analyses suggest that multiple mechanisms drive microbial succession after cholera, including bacterial dispersal properties, changing enteric oxygen and carbohydrate levels, and phage dynamics. Thus, gut microbiota recovery after cholera may be predictable at the level of community structure but is driven by a complex set of temporally varying ecological processes. Our findings suggest opportunities for diagnostics and therapies targeting the gut microbiota in humans recovering from infectious diarrhea.

IMPORTANCE: Disability after diarrhea is a major burden on public health in the developing world. Gut bacteria may affect this recovery, but it remains incompletely understood how resident microbes in the digestive tract respond to diarrheal illness. Here, we observed an orderly and reproducible succession of gut bacterial groups after cholera in humans. Genomic analyses associated the succession with bacterial dispersal in food, an altered microbial environment, and changing phage levels. Our findings suggest that it may one day be feasible to manage resident bacterial populations in the gut after infectious diarrhea.

DOI10.1128/mBio.00381-15
Alternate JournalMBio
PubMed ID25991682
PubMed Central IDPMC4442136
Grant ListP50 GM068763 / GM / NIGMS NIH HHS / United States
R01 AI03055 / AI / NIAID NIH HHS / United States
R01 AI103055 / AI / NIAID NIH HHS / United States
R01 AI106878 / AI / NIAID NIH HHS / United States
R56 AI106878 / AI / NIAID NIH HHS / United States
T32 AI007061 / AI / NIAID NIH HHS / United States
T32 AI070611976 / AI / NIAID NIH HHS / United States
U01 AI058935 / AI / NIAID NIH HHS / United States
U01 AI058935 / AI / NIAID NIH HHS / United States
U01 AI106878 / AI / NIAID NIH HHS / United States