Viewing the human microbiome through three-dimensional glasses: integrating structural and functional studies to better define the properties of myriad carbohydrate-active enzymes.

TitleViewing the human microbiome through three-dimensional glasses: integrating structural and functional studies to better define the properties of myriad carbohydrate-active enzymes.
Publication TypeJournal Article
Year of Publication2010
AuthorsTurnbaugh, PJ, Henrissat, B, Gordon, JI
JournalActa Crystallogr Sect F Struct Biol Cryst Commun
Volume66
IssuePt 10
Pagination1261-4
Date Published2010 Oct 1
ISSN1744-3091
KeywordsBacterial Proteins, Carbohydrate Metabolism, Genomics, Humans, Metagenome, Protein Conformation
Abstract

Recent studies have provided an unprecedented view of the trillions of microbes associated with the human body. The human microbiome harbors tremendous diversity at multiple levels: the species that colonize each individual and each body habitat; the genes that are found in each organism's genome; the expression of these genes and the interactions and activities of their protein products. The sources of this diversity are wide-ranging and reflect both environmental and host factors. A major challenge moving forward is defining the precise functions of members of various families of proteins represented in our microbiomes, including the highly diverse carbohydrate-active enzymes (CAZymes) involved in numerous biologically important chemical transformations, such as the degradation of complex dietary polysaccharides. Coupling metagenomic analyses to structural genomics initiatives and to biochemical and other functional assays of CAZymes will be essential for determining how these as well as other microbiome-encoded proteins operate to shape the properties of microbial communities and their human hosts.

DOI10.1107/S1744309110029088
Alternate JournalActa Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
PubMed ID20944220
PubMed Central IDPMC2954214
Grant ListDK30292 / DK / NIDDK NIH HHS / United States
DK78669 / DK / NIDDK NIH HHS / United States
R37 DK030292 / DK / NIDDK NIH HHS / United States