Xenobiotics shape the physiology and gene expression of the active human gut microbiome.

TitleXenobiotics shape the physiology and gene expression of the active human gut microbiome.
Publication TypeJournal Article
Year of Publication2013
AuthorsMaurice, CFerrier, Haiser, HJoseph, Turnbaugh, PJames
JournalCell
Volume152
Issue1-2
Pagination39-50
Date Published2013 Jan 17
ISSN1097-4172
KeywordsAnti-Bacterial Agents, Feces, Gastrointestinal Tract, Gene Expression Profiling, Gram-Positive Bacteria, Humans, Metagenome, Metagenomics, Xenobiotics
Abstract

The human gut contains trillions of microorganisms that influence our health by metabolizing xenobiotics, including host-targeted drugs and antibiotics. Recent efforts have characterized the diversity of this host-associated community, but it remains unclear which microorganisms are active and what perturbations influence this activity. Here, we combine flow cytometry, 16S rRNA gene sequencing, and metatranscriptomics to demonstrate that the gut contains a distinctive set of active microorganisms, primarily Firmicutes. Short-term exposure to a panel of xenobiotics significantly affected the physiology, structure, and gene expression of this active gut microbiome. Xenobiotic-responsive genes were found across multiple bacterial phyla, encoding antibiotic resistance, drug metabolism, and stress response pathways. These results demonstrate the power of moving beyond surveys of microbial diversity to better understand metabolic activity, highlight the unintended consequences of xenobiotics, and suggest that attempts at personalized medicine should consider interindividual variations in the active human gut microbiome.

DOI10.1016/j.cell.2012.10.052
Alternate JournalCell
PubMed ID23332745
PubMed Central IDPMC3552296
Grant ListMFE-112991 / / Canadian Institutes of Health Research / Canada
P50 GM068763 / GM / NIGMS NIH HHS / United States
P50 GM068763 / GM / NIGMS NIH HHS / United States